Phytotoxicity effect of Silver nanoparticles on Oryza sativa

Phytotoxicity on Oryza sativa was studied by directly exposing it to silver nanoparticles solutions. Seedlings were allowed to grow at room temperature in Hoagland’s nutrient solutions prior to treatment. Particles were prepared in distilled water by Chemical reductions method. U.V-Vis spectra analysis confirms its formations at 400 nm wavelength. 12 days of phytotoxicity periods in 1000μg/ml concentrations of silver nanoparticles leads to adverse effects on plant species. Analysis of particles was initially determined by XRD spectrophotometer and was found to be 25 nm in size. FTIR spectrophotometer results shows that there is change in intensity and shifts in positions of peak in treated roots as compared to control sample. Slight variations in peaks and its intensity of Oryza sativa roots confirm involvement of functional group such as carboxyl, Amine, hydroxyl etc in nanoparticle binding. TEM images revealed that various particle sizes deposited inside the root cells. Interestingly, it was found that during penetrations of particles inside the roots cell, they damaged the cell wall as well as vacuoles to enter. It may be due to the penetrations of large particles entering through small pores of cell walls. This study directly tells the relevant of effects of silver nanoparticles inside cellular mechanisms during phytotoxicity periods.